Development and characterization of 33 SNP markers in a critically endangered Hynobius amjiensis using ddRAD sequencing

Hynobius amjiensis was considered as one of the 29 most threatened amphibian in China. To effectively conserve, manage and recover population this critically endangered species, 33 single nucleotide polymorphism (SNP) markers were developed using double digest restriction-site associated DNA (ddRAD) sequencing. The minor allele frequency per locus ranged from 0.0161 to 0.5000. observed heterozygosity expected varied 0.0323 0.6667 (average 0.3303) 0.0317 0.5000 0.2772), respectively. inbreeding coefficient value between − 0.3315 0.0000. No significant deviation Hardy–Weinberg equilibrium (P > 0.05) found all loci. These novel SNPs will be helpful for genetic assessment conservation H. amjiensis.